Mushroom Research Centre GmbH MRCA GmbH.
  
 
 My Account  |  Instructions  |  Books  |  Videos  |  Newsarchiv  |  Trade&Comm.Purchase  |  Checkout   
  Top » Catalog
Categories
Information
Grow Instructions

Mycorrhizae

Videos

Books


Contact us

Imprint

Driving Directions


Conditions of payment

Shipping & Returns

Conditions of Use

Privacy Notice

FAQ
Reviews more
3 Mushroom Mega Deluxe Growkit
I make you rich so i want to help me ASAP. Hey don't dela ..
5 of 5 Stars!
Affiliate Program
MRCA_affiliate

Affiliate Log In
Affiliate Information
Links
Partner Links


Instruction for
Mycelium germination from spores



You can very easiely germinate spores on an agar medium. The so growen mycelium is called "Multi-Culutre" as opposite to a clone. With this methode of mycelium germination you will not get genetic identical copies of a mushroom culture - form the spores there will grow new organism of the same species. Mainly self made spore prints may contaminted with any bakteria, so we recommend to use antibiotic agar medium for spore germination. It is also possible to make mycelium like described in this instruction form spore syringes.


Recommended Tools:
(for product information, please click on product name)

Spores (Sporeprint or Sporesyringe)
Agar medium in petri dishes
Oneway Inoculation loop or Needelholder with inoculation loop
Parafilm to seal the petris
Spiritus Lamp
Facemask and Hairnet
Latex gloves
Workspace desinfection
Handdesinfection
Glove Bag (sterile workspace)


For detailed instructions how to produce agar medium in petri disches by your self, please click here!


General Procedere:

To keep other microorganism (bakteria, mold,...) away from the agar medium, we recommend to use a HEPA-filter or a Glovebox / Inoculationbox. Spring clean and desinfect workspace and your hands. For reduction of contamination risk it is recommended to wear hearnet, facemask and latex gloves.





Take the inoculation loop and rub it over the sproreprint to grab a few spores (rub till the loop gets a black spores veil). If you use reusable metal inouculation loop, you have to heat it over a spiritus lamp (put it into the flame till the forefront begins to glow). Let it cool down to room temperature bevor bringing in contact with the spores. Do not touch the spores, inoculation loop or agar medium with your fingers or bring in contact with any contamination resource!





Now dispens the spores you have grabed with the inoculation loop on the agar medium. Draw with the loop a "S" from spores on to the agar medium. To reduce contamination risk please work fast and open the petri dish only as far as it is needful to get the inoculation loop in. Seal the petri dishes immediately with parafilm after you have dispensed the spores. If you use spore syringes, please dispens a few drops (1-2 ml) of spore solution on each petri dish.





We reccomend to make about 5 petri dishes from each spore print, because not all spores will germinate equal. During the mycelium grows from the spores, store the petri dishes at the right temperature according to the growing conditions for the species you cultivate. After a few days up to 1 week the spore germination will get as strong that you can see it with the naked eye.

For optimal growth we recommend to select the rhizomorph mycelium strains, before you inoculate grain or substrate with the mycelium.

For detailed instructions with pictures how to do strain selection,
please click here!
New Products For September
Comming soon!!!!
Comming soon!!!!
0.00EUR		 Agaricus bisporus var. albidus | White Button Spore Syringe
Agaricus bisporus var. albidus | White Button Spore Syringe
14.90EUR		 Agaricus bisporus var. avellaneous | Cream Button Spore Syringe
Agaricus bisporus var. avellaneous | Cream Button Spore Syringe
14.90EUR
AOS U7146 Travel Ultrasonic Humidification System
AOS U7146 Travel Ultrasonic Humidification System
49.90EUR		 *Organic Himematsutake dried 50 g
*Organic Himematsutake dried 50 g
16.90EUR		 *Organic Himematsutake dried 25 g
*Organic Himematsutake dried 25 g
8.90EUR
Shopping Cart more
0 items
Languages
English Deutsch
Google™ translate
Euro-Label Seal of Quality
Guetezeichen-Verifikation

Bio Austria

Organic control station: AT-T-01Bio, BIKO Tyrol

Biokontrollstelle:
 AT-T-01Bio, BIKO Tirol
Specials more
Petridish GLASS 18 pcs. Box
Petridish GLASS 18 pcs. Box
59.59EUR
34.96EUR
Bestsellers
*Spore Syringe Pack - 10 x Exotic *Spore Syringe Pack - 10 x Exotic

Antibiotic Malt Extract Agar in Petri 15 pcs. Antibiotic Malt Extract Agar in Petri 15 pcs.

3 Mushroom Mega Deluxe Growkit 3 Mushroom Mega Deluxe Growkit

Shiitake Extract 240 pcs. Shiitake Extract 240 pcs.

Ryegrain Substrate bag 2,7 kg | 4,5 l Ryegrain Substrate bag 2,7 kg | 4,5 l

Bazillol Workspace Desinfect 50ml Bazillol Workspace Desinfect 50ml

Stropharia rugoso annulata | King Stropharia | Spore Syringe Stropharia rugoso annulata | King Stropharia | Spore Syringe

Mycorrhiza Granular 200 g Mycorrhiza Granular 200 g

Ganoderma lucidum | Reishi Fruiting Bag Ganoderma lucidum | Reishi Fruiting Bag

Hypsizygus ulmarius | Elm Oyster | Shirotamogitake Spawn-Dowels Hypsizygus ulmarius | Elm Oyster | Shirotamogitake Spawn-Dowels
What's New? more


98.00EUR
Who's online?
There currently are
 56 guests and
 11 members
 online.
  Thursday 02 September, 2010                                     Informationen zu E-Commerce und Mediengesetz   10184370 requests since Saturday 12 May, 2007  


Copyright © 2007−2009 Mushroom Research Centre MRCA GmbH